The second edition of this best-selling book helps researchers, teachers, and students understand and use the major techniques and methods employed in cellular and molecular biology today. Each chapter covers the principles behind the various methods and techniques, and provides a detailed step-by-step description of each protocol, as well as applications of the protocol to different systems. In addition to presenting new protocols in all chapters, Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes in vitro transcription methods, analysis of DNA sequences, and new bioseparation techniques. Several new chapters cover mRNA differential display, in situ hybridization, cellular and subcellular localization of proteins, combinatorial chemistry, and data mining.