This essential handbook guides researchers in the theory, applications, and practical use of affinity chromatography. It explores preparative and analytical applications of affinity methods as well as recent developments in the use of affinity chromatography with other separation techniques and in combination with biosensors, mass spectometry, or microanalytical systems. This edition has been extensively revised and features new and expanded discussions on topics such as new ligands, recently developed applications, and affinity-based immunossays. It also discusses applications ranging from clinical and pharmaceutical analysis to biotechnology and environmental testing.